software version 7.1.3.0 (Bioedit Company)
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Software Version 7.1.3.0, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae)"
Article Title: Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae)
Journal: Insects
doi: 10.3390/insects15120942
Figure Legend Snippet: Stability rankings of the ten candidate reference genes in Epicauta gorhami computed by the ΔCt method in different samples. The stability values are showed from the least ( left ) to the most stable gene ( right ). Abbreviation: ACT, actin; SOD, superoxide dismutase; α-TUB, α-tubulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EF1α, elongation factor 1α; RPL4, RPL13, RPL27, RPS18 and RPS28, ribosomal protein. The abbreviations are exactly the same as Figures 2–5.
Techniques Used:
Figure Legend Snippet: Stability rankings of the ten candidate reference genes in Epicauta gorhami computed by geNorm, NormFinder and BestKeeper at various adult ages. Diverse ages of Epicauta gorhami adults were sampled (collected on the first to third days of the newly emerged adults). The stability values are showed from the least stable ( left ) to the most stable gene ( right ). ( A , B ) geNorm, ( C ) NormFinder and ( D ) BestKeeper.
Techniques Used:
Figure Legend Snippet: The most optimal reference genes in E. gorhami for various experimental backgrounds.
Techniques Used:
Figure Legend Snippet: Stability rankings of the ten candidate reference genes in Epicauta gorhami calculated by geNorm, NormFinder and BestKeeper among diverse adult tissues. Foregut, midgut, hindgut, epidermis, trachea and antenna were dissected from the 5-day-old adults. The stability values are showed from the least stable ( left ) to the most stable gene ( right ). ( A , B ) geNorm, ( C ) NormFinder and ( D ) BestKeeper.
Techniques Used:
Figure Legend Snippet: Stability rankings of the ten candidate reference genes in Epicauta gorhami assessed by geNorm, NormFinder and BestKeeper at different temperatures. The newly emerged adults reared for 6 h under three temperatures (4 °C, 25 °C and 37 °C) were collected. The stability values are showed from the least stable ( left ) to the most stable gene ( right ). ( A , B ) geNorm, ( C ) NormFinder and ( D ) BestKeeper.
Techniques Used:
Figure Legend Snippet: Stability rankings of the ten candidate reference genes in Epicauta gorhami computed by the Geomean method of RefFinder in different samples. The stability values are showed from the most stable ( left ) to the least stable gene ( right ). ( A ) adult ages, ( B ) adult tissues, ( C ) temperature and ( D ) all samples.
Techniques Used:
Figure Legend Snippet: Relative expression level of EgUAP among diverse adult tissues of Epicauta gorhami . The relative gene expression level of EgUAP in the foregut (FG), midgut (MG), hindgut (HG), epidermis (EP), trachea (TR) and antenna (AN) was normalized to the most suitable reference genes ( RPS18 and RPS28 ) by the 2 −∆∆Ct method. The relative transcripts are the ratios of copy numbers in different adult tissues relative to the epidermis, which is set at 1. The values are means + SE. Different letters indicate significant differences in gene expression among different tissues ( p < 0.05).
Techniques Used: Expressing, Gene Expression